Clitocybin D, a novel human neutrophil elastase inhibitor from the culture broth of Clitocybe aurantiaca.
نویسندگان
چکیده
Clitocybin D, a novel human neutrophil elastase inhibitor, was isolated from the culture broth of Clitocybe aurantiaca. This compound was purified by solvent extraction, silica gel column chromatography, Sephadex LH-20 column chromatography, and preparative HPLC. The compound was determined to be 4-(4,6-dihydroxy-3-methoxy-3H-isoindol-1-yl)-benzoic acid on the basis of 1D and 2D NMRs and MS spectroscopic analysis. Analysis of the human neutrophil elastase (HNE) inhibitory activity of the isolated compound revealed that it showed significant HNE inhibitory activity with an IC(50) value of 17.8 micronM.
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ورودعنوان ژورنال:
- Journal of microbiology and biotechnology
دوره 19 10 شماره
صفحات -
تاریخ انتشار 2009